Can you quantify my samples?

Can you quantify my samples?

Yes, if you would like any of your samples quantified, please indicate which samples you would like us to quantify in the notes section of your order form. We CANNOT quantify crude PCR products, or […]

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What should the sample concentration be?

The sample type will determine the concentration needed. i. PCR Product: 25-50 ng/µl ii. Plasmid DNA: minimum of 50 ng/µl but we prefer it at 100 ng/µl. If you are sending it at 100ng/µl we […]

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Can I premix the sample and primer?

Yes. Be sure you have a total of 20 picomoles of primer per 15µl of premix. We recommend using the following formula to create pre-mix samples: DNA: N=0.1 x (vector size in base pairs + […]

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What volume of primer should I send?

For individual samples, a minimum of 10µl should be sent. If you are using the same primer on multiple samples, we require just 3µl multiplied by the number of reactions you are doing with that […]

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