Yes, if you would like any of your samples quantified, please indicate which samples you would like us to quantify in the notes section of your order form. We CANNOT quantify crude PCR products, or […]
Learn MoreSample Requirements
Can you do primer synthesis?
Yes. It is $16 per primer for standard primers. If you want us to design and synthesize the primer the cost is $25 per primer.
Learn MoreWhat primers do you offer for free?
Please refer to the primers page on the website for a complete list.
Learn MoreDo I have to send my own primer?
No. We have a list of free primers. We can also synthesize and/or design your primers for you. Primers are stored for a period of 2 years.
Learn MoreI am sending cultured pellets and am unsure if they are sufficient. Do you have any suggestions?
If you can check the O.D. of the solution before pelleting, it should be around 3.0-4.0. If you did not check the O.D. prior to pelleting , feel free to send the pellets and, if […]
Learn MoreWhat should the sample concentration be?
The sample type will determine the concentration needed. i. PCR Product: 25-50 ng/µl ii. Plasmid DNA: minimum of 50 ng/µl but we prefer it at 100 ng/µl. If you are sending it at 100ng/µl we […]
Learn MoreCan I premix the sample and primer?
Yes. Be sure you have a total of 20 picomoles of primer per 15µl of premix. We recommend using the following formula to create pre-mix samples: DNA: N=0.1 x (vector size in base pairs + […]
Learn MoreWhat volume of primer should I send?
Primers should be provided at a concentration of 10µM (picomoles/µl). For 96-well format, provide at least 50 µl of primer in a tube for each full plate. For primer plates, provide at least 6ul of […]
Learn MoreWhat concentration should my primer be?
All primers should be sent at 10µM.
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