Standard Primers

Primers on the Standard Primer List (below) are provided free of charge.

Customer Provided Primers

  • Primers should be provided in nuclease free water.
  • Primers should be provided at a concentration of 10µM (picomoles/µl).
  • For 96-well format, provide at least 120 µl of primer for each plate.
  • For individual samples, provide 10 µl per sample.
  • By default, we will run your reactions at an annealing temperature of 50ºC.*

*If you think your primers require a different annealing temperature, make sure to note that on your submission form. This request does cause a delay in results by 24 hours.

Ordering Custom Primers

We can provide custom primers for you, however, it usually adds one additional business day to your turnaround time. Please contact us to get prices for custom primers.

Standard Primer List

M13 Forward (-20)GTA AAA CGA CGG CCA GT
M13 Forward (-41) CGC CAG GGT TTT CCC AGT CAC GAC
M13 Reverse (-27)CAG GAA ACA GCT ATG AC
M13 Reverse (-48)AGC GGA TAA CAA TTT CAC ACA GG
SP6 PromoterGCT ATT TAG GTG ACA CTA TAG
SP6 UpstreamATT TAG GTG ACA CTA TAG
T3 PromoterAAT TAA CCC TCA CTA AAG GG
T7 PromoterTAA TAC GAC TCA CTA TAG GG
T7 TerminatorGCT AGT TAT TGC TCA GCG G
ITS1-FCTT GGT CAT TTA GAG GAA GTA A
ITS4TCC TCC GCT TAT TGA TAT GC
BGH ReverseTAG AAG GCA CAG TCG AGG
CMVFCGC AAA TGG GCG GTA GGC GTG
-96gIIICCC TCA TAG TTA GCG TAA CTG
pcDNA3_forGGC TAA CTA GAG AAC CCA CTG
pcDNA3_revGGC AAC TAG AAG GCA CAG TC
pGex forATA GCA TGG CCT TTG CAG G
pGex revGAG CTG CAT GTG TCA GAG G
pJET1,2forCGA CTC ACT ATA GGG AGA GCG GC
pJET1,2revAAG AAC ATC GAT TTT CCA TGG CAG
pJET1_fwdGCC TGA ACA CCA TAT CCA TCC
pJET1_revGCA GCT GAG AAT ATT GTA GGA GAT C
pQE forGTA TCA CGA GGC CCT TTC GTC T
pQE revCAT TAC TGG ATC TAT CAA CAG GAG
SL1CAG TCC AGT TAC GCT GGA GTC
SR2GGT CAG GTA TGA TTT AAA TGG TCA GT
27FAGA GTT TGA TCM TGG CTC AG
1492RGGT TAC CTT GTT ACG ACT T
16s-R(533R)TAC CGC GGC TGC TGG CAC
pGLforGTA TCT TAT GGT ACT GTA ACT G
pGLrevCTT TAT GTT TTT GGC GTC TTC C
Neo-FCGT TGG CTA CCC GTG ATA TT
Neo-RTGG ATA CTT TCT CGG CAG
pBAD ForwardATG CCA TAG CAT TTT TAT CC
pBAD ReverseGAT TTA ATC TGT ATC AGG
EGFP-CCAT GGT CCT GCT GGA GTT CGT G
EGFP-NCGT CGC CGT CCA GCT CGA CCA G
V5ACC GAG GAG AGG GTT AGG GAT