Standard Primers

Primers on the Standard Primer List (below) are kept in our in-house stock for use free of charge.

Customer Provided Primers

  • Primers should be provided at a concentration of 10µM (picomoles/µl).
  • For 96-well format, provide at least 50 µl of primer in a tube for each full plate.
  • For primer plates, provide at least 6ul of primer per well of the plate.
  • For individual samples, provide 2 µl of primer per sequencing reaction + 5 µl.
    • Example: 4 sequencing reactions = 8 µl + 5 = 13 µl total primer to send.

Ordering Custom Primers

If you would like custom primers made for your order, select the ‘Synthesize’ option in the primer column when placing your order. We will keep any custom primers for 2 years from the date of synthesis. Ordering custom primers will add 1-2 additional business days to your order turnaround time.

Primer Design Service

If you would like us to design primers for your project, select the ‘Synthesize’ option on your order form in the primer column when placing your order. Please contact us ahead of time through email to discuss the project and to provide reference sequence information. If you do not have a full reference sequence, we can primer walk through your sample with a known starting point for sequencing.

Standard Primer List

Downloadable list of primers

Primer NameSequence (5' → 3')
M13 Forward (-20)GTA AAA CGA CGG CCA GT
M13 Forward (-41) CGC CAG GGT TTT CCC AGT CAC GAC
M13 Reverse (-27)CAG GAA ACA GCT ATG AC
M13 Reverse (-48)AGC GGA TAA CAA TTT CAC ACA GG
SP6 PromoterGCT ATT TAG GTG ACA CTA TAG
SP6 UpstreamATT TAG GTG ACA CTA TAG
T3 PromoterAAT TAA CCC TCA CTA AAG GG
T7 PromoterTAA TAC GAC TCA CTA TAG GG
T7 TerminatorGCT AGT TAT TGC TCA GCG G
ITS1-FCTT GGT CAT TTA GAG GAA GTA A
ITS4TCC TCC GCT TAT TGA TAT GC
BGH ReverseTAG AAG GCA CAG TCG AGG
CMVFCGC AAA TGG GCG GTA GGC GTG
-96gIIICCC TCA TAG TTA GCG TAA CTG
pcDNA3_forGGC TAA CTA GAG AAC CCA CTG
pcDNA3_revGGC AAC TAG AAG GCA CAG TC
pGex forATA GCA TGG CCT TTG CAG G
pGex revGAG CTG CAT GTG TCA GAG G
pJET1,2forCGA CTC ACT ATA GGG AGA GCG GC
pJET1,2revAAG AAC ATC GAT TTT CCA TGG CAG
pJET1_fwdGCC TGA ACA CCA TAT CCA TCC
pJET1_revGCA GCT GAG AAT ATT GTA GGA GAT C
pQE forGTA TCA CGA GGC CCT TTC GTC T
pQE revCAT TAC TGG ATC TAT CAA CAG GAG
SL1CAG TCC AGT TAC GCT GGA GTC
SR2GGT CAG GTA TGA TTT AAA TGG TCA GT
27FAGA GTT TGA TCM TGG CTC AG
1492RGGT TAC CTT GTT ACG ACT T
16s-R(533R)TAC CGC GGC TGC TGG CAC
pGLforGTA TCT TAT GGT ACT GTA ACT G
pGLrevCTT TAT GTT TTT GGC GTC TTC C
Neo-FCGT TGG CTA CCC GTG ATA TT
Neo-RTGG ATA CTT TCT CGG CAG
pBAD ForwardATG CCA TAG CAT TTT TAT CC
pBAD ReverseGAT TTA ATC TGT ATC AGG
EGFP-CCAT GGT CCT GCT GGA GTT CGT G
EGFP-NCGT CGC CGT CCA GCT CGA CCA G
V5ACC GAG GAG AGG GTT AGG GAT