Standard Primers
Primers on the Standard Primer List (below) are provided free of charge.
Customer Provided Primers
- Primers should be provided in nuclease free water.
- Primers should be provided at a concentration of 10µM (picomoles/µl).
- For 96-well format, provide at least 120 µl of primer for each plate.
- For individual samples, provide 10 µl per sample.
- By default, we will run your reactions at an annealing temperature of 50ºC.*
*If you think your primers require a different annealing temperature, make sure to note that on your submission form. This request does cause a delay in results by 24 hours.
Ordering Custom Primers
We can provide custom primers for you, however, it usually adds one additional business day to your turnaround time. Please contact us to get prices for custom primers.
Standard Primer List
| M13 Forward (-20) | GTA AAA CGA CGG CCA GT |
| M13 Forward (-41) | CGC CAG GGT TTT CCC AGT CAC GAC |
| M13 Reverse (-27) | CAG GAA ACA GCT ATG AC |
| M13 Reverse (-48) | AGC GGA TAA CAA TTT CAC ACA GG |
| SP6 Promoter | GCT ATT TAG GTG ACA CTA TAG |
| SP6 Upstream | ATT TAG GTG ACA CTA TAG |
| T3 Promoter | AAT TAA CCC TCA CTA AAG GG |
| T7 Promoter | TAA TAC GAC TCA CTA TAG GG |
| T7 Terminator | GCT AGT TAT TGC TCA GCG G |
| ITS1-F | CTT GGT CAT TTA GAG GAA GTA A |
| ITS4 | TCC TCC GCT TAT TGA TAT GC |
| BGH Reverse | TAG AAG GCA CAG TCG AGG |
| CMVF | CGC AAA TGG GCG GTA GGC GTG |
| -96gIII | CCC TCA TAG TTA GCG TAA CTG |
| pcDNA3_for | GGC TAA CTA GAG AAC CCA CTG |
| pcDNA3_rev | GGC AAC TAG AAG GCA CAG TC |
| pGex for | ATA GCA TGG CCT TTG CAG G |
| pGex rev | GAG CTG CAT GTG TCA GAG G |
| pJET1,2for | CGA CTC ACT ATA GGG AGA GCG GC |
| pJET1,2rev | AAG AAC ATC GAT TTT CCA TGG CAG |
| pJET1_fwd | GCC TGA ACA CCA TAT CCA TCC |
| pJET1_rev | GCA GCT GAG AAT ATT GTA GGA GAT C |
| pQE for | GTA TCA CGA GGC CCT TTC GTC T |
| pQE rev | CAT TAC TGG ATC TAT CAA CAG GAG |
| SL1 | CAG TCC AGT TAC GCT GGA GTC |
| SR2 | GGT CAG GTA TGA TTT AAA TGG TCA GT |
| 27F | AGA GTT TGA TCM TGG CTC AG |
| 1492R | GGT TAC CTT GTT ACG ACT T |
| 16s-R(533R) | TAC CGC GGC TGC TGG CAC |
| pGLfor | GTA TCT TAT GGT ACT GTA ACT G |
| pGLrev | CTT TAT GTT TTT GGC GTC TTC C |
| Neo-F | CGT TGG CTA CCC GTG ATA TT |
| Neo-R | TGG ATA CTT TCT CGG CAG |
| pBAD Forward | ATG CCA TAG CAT TTT TAT CC |
| pBAD Reverse | GAT TTA ATC TGT ATC AGG |
| EGFP-C | CAT GGT CCT GCT GGA GTT CGT G |
| EGFP-N | CGT CGC CGT CCA GCT CGA CCA G |
| V5 | ACC GAG GAG AGG GTT AGG GAT |
