The sample type will determine the concentration needed.
i. PCR Product: 25-50 ng/µl
ii. Plasmid DNA: minimum of 50 ng/µl but we prefer it at 100 ng/µl. If you are sending it at 100ng/µl we need 10µl for one reaction or 15µl for two reactions. If you are sending it at less than 100ng/µl please send 15µl for one reaction or 25µl for two reactions.
*Note: If your samples are below the desired concentrations as listed above, we can still sequence them, the results are just less likely to be of high-quality. Please indicate the concentration on your order form if known so that we can adjust the sample volume to add per reaction accordingly.