Samples sent as Crude PCR products will be cleaned using our Exo/Sap protocol and then DNA will be sequenced. The prices are as follows:
| 96-well Plates | $360 per plate (Add'l reads are $360/plate) |
| Individual Samples | $8.50 per sample (Add'l reads are $7/sample) |
Preparing Crude PCR Products
To avoid any degradation, it is important that your samples are frozen immediately after amplification. Crude PCR products should be provided in a total volume of about 10µL and concentrated per the chart below. Feel free to use this Dilution Calculator to help get the right concentration.
| Length of PCR amplicon | Desired ng/µL |
|---|---|
| 100-200 bp | 5 |
| 200-500 bp | 5-10 |
| 500-1000 bp | 10-20 |
| 1000-2000 bp | 20-40 |
| >2000 bp | 50 |
Shipping Crude PCR Products
Please ship overnite on dry ice. If possible, seal the plate with foil tape. Some plastic tapes lose their seal when shipped on dry ice.
Please enclose a printed copy of your Sample Submission Form and ship (via any carrier) to:
Functional Biosciences, Inc.
505 South Rosa Road
Suite 238
Madison, WI 53719
Please note that samples are not delivered to our facilities on Saturday or Sundays.