What volume of primer should I send?

  • Primers should be provided at a concentration of 10µM (picomoles/µl).
  • For 96-well format, provide at least 50 µl of primer in a tube for each full plate.
  • For primer plates, provide at least 6ul of primer per well of the plate.
  • For individual samples, provide 2 µl of primer per sequencing reaction + 5 µl.
    • Example: 4 sequencing reactions = 8 µl + 5 = 13 µl total primer to send.