Full Plasmid Sequencing (New!)

 

Sequence full plasmids up to 25kb in size in a single run using Oxford Nanopore technology!

Sanger Sequencing & PCR

 

Purified Plasmid DNA
Isolated DNA in plasmids that have undergone transformation and then plasmid purification.
 
Purified PCR Products
Sample that has undergone a PCR reaction and cleanup protocol to remove excess dNTPs and oligonucleotides.
 
Premixed DNA and Primer
Either purified plasmid or PCR products already mixed with primer.
Crude PCR Products
PCR amplified DNA that requires cleanup before sequencing.
 
Genomic DNA for PCR
Isolated DNA ready for PCR amplification and sequencing.

Plasmid Growth & Purification

 

Agar Plates – Bacterial Colonies
Transformed bacterial colonies grown on agar plates containing antibiotic resistance.
 
Glycerol Stocks
Suspended bacterial cultures in a stabilized glycerol stock solution.
Pelleted Cultures
Bacterial cultures grown and pelleted in deep well plates.

Capillary Electrophoresis

 

Ready to Inject
Samples that have undergone the Big Dye reaction needing electrophoresis on the ABI instrument either with or without cleanup.
Fragment Analysis
Fragmented DNA labeled with fluorescent dye that undergoes capillary electrophoresis.