Primers

Standard Primers

Primers on the Standard Primer List (below) are kept in our in-house stock for use free of charge.

Customer Provided Primers

• Primers should be provided at a concentration of 10µM (picomoles/µl).
• For 96-well format, provide at least 50 µl of primer in a tube for each full plate.
• For primer plates, provide at least 6ul of primer per well of the plate.
• For individual samples, provide 2 µl of primer per sequencing reaction + 5 µl.
  • Example: 4 sequencing reactions = 8 µl + 5 = 13 µl total primer to send.

Ordering Custom Primers

If you would like custom primers made for your order, select the ‘Synthesize’ option in the primer column when placing your order. We will keep any custom primers for 2 years from the date of synthesis. Ordering custom primers will add 1-2 additional business days to your order turnaround time.

Primer Design Service

If you would like us to design primers for your project, select the ‘Synthesize’ option on your order form in the primer column when placing your order. Please contact us ahead of time through email to discuss the project and to provide reference sequence information. If you do not have a full reference sequence, we can primer walk through your sample with a known starting point for sequencing.

Standard Primer List

Downloadable list of primers