Sequencing Results

How do I receive the results?

We will send you an email when your results are posted to the website. You can then login and download your results.

Will you keep my samples and/or primers?

We will hold on to any samples and primers sent with an order for 5 weeks.

If we synthesize a primer for you, we will put it in our long-term storage which is kept for two years. If requested, we can hold onto it for longer while replenishing the stock as needed.

Can I get my samples back?

Yes. We will ship them to you if requested; however, we will add the shipping charge to your invoice for this service.

Can I submit a new order and request to use samples I previously sent to you?

Yes. Please indicate in the notes section of your order form if the samples or primers come from a previous order. You can call or email to be sure we still have the samples you are requesting at a sufficient volume.

How long are results available on my account?

Results are available for six months from the date they are posted.

What are the 5 txt files that come with my results?

Below is an explanation of each type of file we provide.

.ab1 Files: The .ab1 file is the chromatogram (trace) file from your sample. By viewing this file type you’ll be able to see the actual peaks associated with each base call. Programs to use: There are a number of different programs that can be used to view these files. We have links to a couple of these, here.

.raw.seq.txt: This is an untrimmed fasta file. This file is not edited and may contain some low quality base calls. It’s important to verify these calls by viewing the .raw.qual.txt quality score file or the peaks in the chromatogram.

.raw.qual.txt: This file lists the phred quality score of each base in the .raw.seq.txt file.
Probability of Error = 10^(-Quality Score/10)
So a quality score of 20+ means there’s less than a 1% chance of error.

seq.txt: This is the trimmed version of the fasta file. In this file, high quality bases with phred scores above 20 are capitalized, low quality bases are in lower case. Low quality bases are trimmed off the start and ends of the sequences. Wells that have no high quality bases are omitted from this file.

seq.qual.txt: This file lists the phred quality score of each base in the trimmed seq.txt file. This file lists information on read lengths and quality scores for each well.