Full Plasmid Sequencing Guidelines

  • To submit your order, download the order submission excel document here: Full Plasmid Sequencing Order Form
    • Fill out the order form and email it to us at sequence@functionalbio.com
    • Send us your samples as you normally do along with a printed version of the Sequencing Order Form, or use our Sample Submission Guidelines page for resources and instructions on shipping and deliveries
    • Make sure you have an account with us to receive your results via our website download
    • Online order submissions coming soon!
  • Submit your purified plasmid up to 25kb in size in either 1.5mL tubes, or strip-tubes.
  • Samples should be quantified with Qubit Fluorometer or similar for most accurate results and best chance of sequencing success. Nanodrop is NOT recommended for quantifying DNA samples and may cause sequencing failure. If you do not have access to a Qubit Fluorometer, we can check your sample concentration for you! Please request this along with your sample submission! We will charge an additional $1.50 per sample for quantification.
  • Sample concentration and volume requirements are as follows:
    • 20-200ng/uL of DNA, submit at least 15uL total volume
    • Over 200ng/uL, submit at least 5uL total volume
  • Avoid high EDTA elution buffers. Water and most commercial kit elution buffers are acceptable.
  • Only numerical, alphabetical, and dash(-) characters are allowed in sample names. Any other characters will be removed during sample processing.
  • Results will be delivered with the following file types:
    • BED
    • GenBank DNA
    • TSV
    • FASTA (Consensus Sequence)
    • FASTQ
    • .html (Plasmid Map)

Benefits of Full Plasmid Sequencing

  • High Accuracy: Delivering precise sequencing results with advanced nanopore technology.
  • Comprehensive Coverage: Providing complete plasmid sequences up to 25kb in a single run, including challenging regions such as high GC.
  • Rapid Turnaround: Ensuring quick delivery of sequencing data to accelerate your research.
  • Cost-Effective: Offering competitive pricing without compromising on quality.