Frequently Asked Questions
Q1. What services do you offer?
We offer Sanger DNA sequencing, PCR, Plasmid Purification, Primer Walking, SNP Analysis, Capillary Electrophoresis, Picking & Growing Colonies, on already sequenced and cleaned up samples (Ready to Inject Plates).
Q2. What is the turnaround time for results?
The turnaround time depends on the number of reactions.
For individual sample orders sent as purified DNA or crude PCR product the turnaround time is normally 24 hours if samples are received by 4pm. Re-sequencing reactions for any reason adds an extra 24 hours approximately (not including weekends).
For plate orders the turnaround time can take anywhere from 48-72 hours. For plate orders containing a large number of plates the turnaround time will be a little longer, but we will provide you the results as we get them done which should start within 72 hours.
It will take one extra day for samples sent as colonies on agar plates for plasmid purification or for pelleted cultures.
Q4. What is the average read length per sample?
All of our reads will read out to over 1000 bases; however we typically get approximately 800 bases of high quality sequence per reaction. The read length will vary depending on sample quality.
Q2. What volume of primer should I send?
For individual samples a minimum of 10µl should be sent. If you are using the same primer on multiple samples, we require just 3µl multiplied by the number of reactions you are doing with that primer, but still a minimum of 10µl.
For full plates we need 100µl of primer at 10µM per plate. If you are sequencing multiple plates that will use the same primer, send 100µl plus 30µl for each additional plate.
Q3. Can I premix the sample and primer?
Yes. Be sure you have a total of 20 picomoles of primer per 15µl of premix. We recommend using the following formula to create pre-mix samples:
DNA: N=0.1 x (vector size in base pairs + insert size in base pairs)
*N is the nanograms of DNA that should be added to each 15µl of premix.
Q4. What should the sample concentration be?
The sample type will determine the concentration needed.
i. PCR Product: 25-50 ng/µl
ii. Plasmid DNA: minimum of 50 ng/µl but we prefer it at 100 ng/µl. If you are sending it at 100ng/µl we need 10µl for one reaction or 15µl for two reactions. If you are sending it at less than 100ng/µl please send 15µl for one reaction or 25µl for two reactions.
Q5. I am sending cultured pellets and am unsure if they are sufficient. Do you have any suggestions?
If you can check the O.D. of the solution before pelleting, it should be around 3.0-4.0. If you did not check the O.D. prior to pelleting , feel free to send the pellets and, if upon arrival they appear too small, we will re-grow them for you at no charge and proceed to contact you to discuss changes for future plates to help ensure proper size.
*If we have to re-grow them this will add an additional day for turnaround time.
Q7. Which carrier should I use for shipment?
Whichever is most convenient for you; we recommend UPS or FedEx for faster shipping. It is best to send the samples priority overnight so we get them by 10:30 a.m. If you send them standard overnight, we may get them in the morning but sometimes we do not get standard overnight shipments until 3 p.m. which can delay the results. If you choose to send samples USPS, please understand that there will be an extra day for turnaround as we do not receive those packages until late in the afternoon.
Q1. What are my payment options?
You can pay by check, credit card, wire transfer, or direct deposit. We accept Visa and MasterCard (online only). We can also set you up with a pre-paid account or automatic payments.
Q3. Do you require a PO on all orders?
No, we do not require a PO. This is up to your institution. If you are required to have a PO by your institution, please indicate the PO number on your sample submission form along with the address that the invoice should be mailed to.