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Standard Primers

     Primers on the Standard Primer list are provided free of charge. 

Customer Provided Primers

• Primers should be provided in nuclease free water.
• Primers should be provided at a concentration of 10µM (picomoles/µl).
• For 96-well format, provide at least 120 µl of primer for each plate.
• For individual samples, provide 10 µl per sample.
• By default, we will run your reactions at an annealing temperature of 50ºC.*

*If you think your primers require a different annealing temperature, make sure to note that on your submission form. If you are unsure of the correct Ta, please include the primer sequence on your submission form.

Ordering Custom Primers

 

Standard Primer List

M13 Forward (-20)	GTA AAA CGA CGG CCA GT
M13 Forward (-41)	CGC CAG GGT TTT CCC AGT CAC GAC
M13 Reverse (-27)	CAG GAA ACA GCT ATG AC
M13 Reverse (-48)	AGC GGA TAA CAA TTT CAC ACA GG
SP6 Promoter	GCT ATT TAG GTG ACA CTA TAG
SP6 Upstream	ATT TAG GTG ACA CTA TAG
T3 Promoter	AAT TAA CCC TCA CTA AAG GG
T7 Promoter	TAA TAC GAC TCA CTA TAG GG
T7 Terminator	GCT AGT TAT TGC TCA GCG G
ITS1-F	CTT GGT CAT TTA GAG GAA GTA A
ITS4	TCC TCC GCT TAT TGA TAT GC
BGH Reverse	TAG AAG GCA CAG TCG AGG
CMVF	CGC AAA TGG GCG GTA GGC GTG
-96gIII	CCC TCA TAG TTA GCG TAA CTG
pcDNA3_for	GGC TAA CTA GAG AAC CCA CTG
pcDNA3_rev	GGC AAC TAG AAG GCA CAG TC
pGex for	ATA GCA TGG CCT TTG CAG G
pGex rev	GAG CTG CAT GTG TCA GAG G
pJET1,2for	CGA CTC ACT ATA GGG AGA GC GCC
pJET1,2rev	AAG AAC ATC GAT TTT CCA TGG CAG
pJET1_fwd	GCC TGA ACA CCA TAT CCA TCC
pJET1_rev	GCA GCT GAG AAT ATT GTA GGA GAT C
pQE for	GTA TCA CGA GGC CCT TTC GTC T
pQE rev	CAT TAC TGG ATC TAT CAA CAG GAG
SL1	CAG TCC AGT TAC GCT GGA GTC
SR2	GGT CAG GTA TGA TTT AAA TGG TCA GT
16s-F	AGA GTT TGA TCC TGG CTC
16s-R	TAC CGC GGC TGC TGG CAC