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FUNCTIONAL BIOSCIENCES DNA SEQUENCING
FUNCTIONAL BIOSCIENCES DNA SEQUENCING
 
 
 

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Primers for DNA Sequencing

Standard Primers

     Primers on the Standard Primer List (below) are provided free of charge. 

Customer Provided Primers

  • Primers should be provided in nuclease free water.
  • Primers should be provided at a concentration of 10µM (picomoles/µl).
  • For 96-well format, provide at least 120 µl of primer for each plate.
  • For individual samples, provide 10 µl per sample.
  • By default, we will run your reactions at an annealing temperature of 50ºC.*
*If you think your primers require a different annealing temperature, make sure to note that on your submission form. If you are unsure of the correct Ta, please include the primer sequence on your submission form. Ordering Custom Primers

We can provide custom primers for you, however, it usually adds one additional business day to your turnaround time. Please contact us to get prices for custom primers.

 

Standard Primer List

M13 Forward (-20)  GTA AAA CGA CGG CCA GT
M13 Forward (-41) CGC CAG GGT TTT CCC AGT CAC GAC
M13 Reverse (-27) CAG GAA ACA GCT ATG AC
M13 Reverse (-48)  AGC GGA TAA CAA TTT CAC ACA GG
SP6 Promoter  GCT ATT TAG GTG ACA CTA TAG
SP6 Upstream ATT TAG GTG ACA CTA TAG
T3 Promoter   AAT TAA CCC TCA CTA AAG GG
T7 Promoter TAA TAC GAC TCA CTA TAG GG
T7 Terminator    GCT AGT TAT TGC TCA GCG G
ITS1-F CTT GGT CAT TTA GAG GAA GTA A
ITS4 TCC TCC GCT TAT TGA TAT GC
BGH Reverse TAG AAG GCA CAG TCG AGG
CMVF CGC AAA TGG GCG GTA GGC GTG
-96gIII CCC TCA TAG TTA GCG TAA CTG
pcDNA3_for GGC TAA CTA GAG AAC CCA CTG
pcDNA3_rev GGC AAC TAG AAG GCA CAG TC
pGex for ATA GCA TGG CCT TTG CAG G
pGex rev GAG CTG CAT GTG TCA GAG G
pJET1,2for CGA CTC ACT ATA GGG AGA GCG GC
pJET1,2rev AAG AAC ATC GAT TTT CCA TGG CAG
pJET1_fwd GCC TGA ACA CCA TAT CCA TCC
pJET1_rev GCA GCT GAG AAT ATT GTA GGA GAT C
pQE for GTA TCA CGA GGC CCT TTC GTC T
pQE rev CAT TAC TGG ATC TAT CAA CAG GAG
SL1 CAG TCC AGT TAC GCT GGA GTC
SR2 GGT CAG GTA TGA TTT AAA TGG TCA GT
27F AGA GTT TGA TCM TGG CTC AG
1492R GGT TAC CTT GTT ACG ACT T
16s-R(533R) TAC CGC GGC TGC TGG CAC
pGLfor GTA TCT TAT GGT ACT GTA ACT G
pGLrev CTT TAT GTT TTT GGC GTC TTC C
Neo-F CGT TGG CTA CCC GTG ATA TT
Neo-R TGG ATA CTT TCT CGG CAG
pBAD Forward ATG CCA TAG CAT TTT TAT CC
pBAD Reverse GAT TTA ATC TGT ATC AGG
EGFP-C CAT GGT CCT GCT GGA GTT CGT G
EGFP-N CGT CGC CGT CCA GCT CGA CCA G
V5 ACC GAG GAG AGG GTT AGG GAT