For Genomic DNA we prefer that the concentration is ~20ng/ul in a total volume of 10ul per well. Primers should be provided at 10uM and we need a minimum volume of 225ul for each primer.
The cost for the PCR, PCR clean-up, and the first DNA sequencing reaction is as follows:
||$480 per plate (add'l reads are $336/plate)
||$9.50 per sample (add'l reads are $7/sample)
Shipping Purified Genomic DNA
Individual samples can be shipped at room temperature without degradation of results as long as the samples are free of contaminants. Be sure that each sample is tightly sealed. For 1.7 ml tubes, many customers like to wrap the tops in parafilm as an additional safeguard.
For 96-well plates, we recommend the following to prevent leakage during shipment:
- Seal the plates tightly. Strip caps are the most reliable option, just make sure the caps and plate are compatible and form a tight seal. If you want to use tape pads we recommend that you use a strong aluminum sealing tape, making sure the tape is firmly sealed around each individual well, and ship on dry ice (see below). In our experience, some plastic tapes can lose their seal when shipped on dry ice.
- Freeze the samples and ship on dry ice. Dry ice will keep the samples frozen during shipment, which should guard against any possible leakage. Cold packs do not offer the same guarantee, especially during the warm summer months. If you are confident that your plate is well sealed, you can send purified plasmids and purified pcr products at room temperature without degrading the results.
- Pack the plates snugly to minimize shifting or jostling during shipment. You may want to use some paper or foil to fill any extra space in the box.
Upon receipt, we’ll check the seals and well volumes to make sure that no leakage is evident.
Please enclose a printed copy of your Sample Submission Form and ship (via any carrier) to:
Functional Biosciences, Inc.
505 South Rosa Road
Madison, WI 53719
**Please note that samples are not delivered to our facilities on Saturday or Sundays.