Pelleted Bacterial Cultures

Samples sent as pelleted cultures will be purified by miniprep then sequenced.  The prices are:  

 

               96-well plates:              $4/sample (additional reads $3/sample)

                                                        $384/plate (additional reads $288/plate)

               Individual Samples:     $9.50/sample (additional reads $7/sample)

 

Preparation of Pelleted Cultures

1. Colonies should be grown in Terrific Broth in "Square Section" Deep-Well Plates. Add 1 ml of Terrific Broth solution     to each well, inoculate with bacteria, then cover with airpore tape.  

2. Grow the cultures in a heated shaker.  Growing conditions vary depending on the type of shaker you are using. Optimal growth is an O.D600 of 1.0-4.0.  With our in-house shakers, which have a 3 mm horizontal-circular orbit, we shake at 800 rpm's for 14.5 hours. 

3. After growth, pellet the cultures by spinning at 1500 x g for 15 minutes.  

4. Dump off the supernatant then pound the block upside down on a paper towel to remove any residual fluid.

5. After supernatant is removed, seal with foil tape to prepare for shipment. Some plastic tapes will lose their seal during shipment on dry ice. Store the blocks at -80C until shipment.

Shipment of Pelleted Cultures

Pelleted cultures should be shipped overnight on dry ice. The samples can be shipped via any carrier you choose to: 

 

Functional Biosciences, Inc.

505 South Rosa Road

Suite 17

Madison, WI 53719

 

Enclose a printed copy of your Sample Submission Form.  Please note that samples are not delivered to our facilities on Saturday or Sundays.