DNA Sequencing Services  
 
 

Submitting Glycerol Stocks

Samples sent as glycerol stocks can enter one of two pipelines.

 

Pipeline 1= PCR: In this pipeline we would receive the samples, do a PCR reaction directly from the stock, purify the PCR reaction and then perform the sequencing reaction. The cost for this is:

         96-well plates:                $5/sample (additional reads $3.50/sample)

                                                    $480/plate (additional reads $336/plate)

         Individual Samples:       $9.50/sample (additional reads $7/sample)

  

Pipeline 2= Plasmid Purification: In this pipeline we would receive the samples, grow a culture from the stock, perform plasmid purification mini-prep and then perform the sequencing reaction. The cost for this is:

 

          96-well plates:               $4.50/sample (additional reads $3/sample)

                                                    $432/plate (additional reads $288/plate)

          Individual Samples:      $10/sample (additional reads $7/sample)

Preparing Glycerol Stocks

After preparation of your glycerol stocks remove a minimum of 15ul into a new 96 well plate or strip-tube for shipment. 

 

Shipping Glycerol Stocks

Glycerol stocks should remain frozen until shipment and should be sent overnight on dry ice, through any carrier of your choice to:

Functional Biosciences, Inc.

505 South Rosa Road

Suite 17

Madison, WI 53719

 

Enclose a printed copy of your sample submission form. Please note that samples are not delivered to our facilities on Saturday or Sundays. 

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