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Functional Biosciences, Inc. |
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| DNA Sequencing Services | |||||
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Submitting Glycerol Stocks
Samples sent as glycerol stocks can enter one of two pipelines.
Pipeline 1= PCR: In this pipeline we would receive the samples, do a PCR reaction directly from the stock, purify the PCR reaction and then perform the sequencing reaction. The cost for this is:
96-well plates: $5/sample (additional reads $3.50/sample)
$480/plate (additional reads $336/plate)
Individual Samples: $9.50/sample (additional reads $7/sample)
Pipeline 2= Plasmid Purification: In this pipeline we would receive the samples, grow a culture from the stock, perform plasmid purification mini-prep and then perform the sequencing reaction. The cost for this is:
96-well plates: $4.50/sample (additional reads $3/sample)
$432/plate (additional reads $288/plate)
Individual Samples: $10/sample (additional reads $7/sample)
Preparing Glycerol Stocks
After preparation of your glycerol stocks remove a minimum of 15ul into a new 96 well plate or strip-tube for shipment.
Shipping Glycerol Stocks
Glycerol stocks should remain frozen until shipment and should be sent overnight on dry ice, through any carrier of your choice to:
Functional Biosciences, Inc.
505 South Rosa Road
Suite 17
Madison, WI 53719
Enclose a printed copy of your sample submission form. Please note that samples are not delivered to our facilities on Saturday or Sundays.
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